Chemical & Biomolecular Engineering

Larsen Lab attends BMES Annual Meeting

The Biomedical Engineering Society (BMES) is an organization focused on advancing human health and well-being. Each year, they host an annual meeting where students, faculty, and industry have the opportunity to connect and present their scientific work. This year, the meeting was hosted in Orlando, Florida from October 6th to the 9th. The Larsen Lab submitted abstracts that were selected for two podium presentations and six poster presentations. Through funding from Creative Inquiry and Dr. Larsen’s grants, a team of 14 students were able to attend. The projects that receiving a coveted podium presentation were led by Ph.D. Student Bipin Paruchuri and undergraduate student team Chlo Forenzo, Danielle LaVigne, and Vaishnavi Kanduri.

Presentations

The first in-person podium presentation ever given by the Larsen Lab was presented by senior ChBE Chlo Forenzo, on Friday October 9th, which is a significant honor for an undergraduate researcher. Her team, comprised of Danielle LaVigne, junior genetics major, Sara Edgecomb, recent ChBE graduate, and Vaishnavi Kanduri, sophomore BioE, looks at the use of polymersomes as Cas9 delivery vehicles as gene knockdown tools to create therapeutic edits.

Minhyun Shin, junior BioE, and Emmeline Bagwell, senior biochemistry major, presented their work on the development of a zebrafish model of Parkinson’s disease and the characterization of new treatment modalities on Friday October 9th.

Undergraduate students, Kayleigh Trumbull and Conner Lumb during their poster presentation.

Two ChBE undergraduate students, Kayleigh Trumbull, sophomore, and Conner Lumb, junior, presented on their SC EPSCoR funded work to characterize most appropriate polymersomes for nerve regeneration applications on Friday October 9th.

A creative inquiry team, Taylor Hamas, senior BioE, Jess Jager, senior ChBE, and Joey Lavalla, senior BioE, co-advised by Dr. Angela Alexander-Bryant in Bioengineering and her PhD student Megan Pitz, presented their work on novel CT imaging agents to enhance glioblastoma detection in a poster on Friday October 9th.

Christopher Pierce, ChBE senior, gave a virtual poster on his work modulating polymersome shape to enhance cellular uptake, leading towards delivery of drugs through the blood-brain barrier.

Morgan Ethridge, junior biochemistry major, presented on behalf of her creative inquiry team on their SC INBRE funded work to determine the validity of enzyme activities as biomarkers of neurodegenerative disease GM1 gangliosidosis on Saturday October 10th.

Ph.D. Student Bipin Paruchuri during podium presentation.

Bipin Paruchuri, 5th year PhD student in ChBE, presented on his work to develop pH and enzyme responsive polymersomes to enhance enzyme replacement therapy in neurodegenerative disease GM1 gangliosidosis on Saturday October 10th. His results were a significant part of Dr. Larsen’s NSF CAREER proposal that was funded earlier this year.

Mark Pitman, fourth year PhD student in ChBE, presented on his work, funded through the CU SEED program, on the development of thermally-responsive, citrate-based hydrogels, as iron chelating biomaterials to improve microenvironment after nerve injury, promoting nerve regeneration on Saturday October 10th.

Not only was great science presented, but the team was able to take a day away from the conference and enjoy their hard work with a trip to Disney’s Animal Kingdom on Thursday October 8th.

On Friday, BMES shut down part of the Universal Islands of Adventure park for their big BMES bash where the team got to enjoy countless rides with no line!

Larsen Lab is grateful to have had the opportunity to present their work and to enjoy time bonding as a team.

 

What are our undergraduates working on this summer?

Dr. Jessica Larsen’s Research Lab –
Grace Anderson, junior undergraduate researcher

Grace Anderson, a junior undergraduate researcher for Dr. Jessica Larsen, is working on an SC BioCRAFT funded project this summer. The overarching goal of Grace’s project is to improve the current standard of care for brain tumors.

Cancerous cells left adjacent to the removed tumor account for 96% of recurrent tumors, providing motivation for immediate local drug delivery to that area while helping the tissue to heal. Preventing tumor resurgence and healing the brain tissue post-surgery can be performed simultaneously using thermally responsive hydrogels.

Grace is working to create polymers that are liquid at room temperature and gel up at body temperature, encapsulating and delivering a drug slowly over time.

Dr. Eric Davis’s Research Lab –

This summer, undergraduate researchers Jaden Stutts and Alana LeSuer are working in Prof. Davis’s lab on highly interdisciplinary projects encompassing polymer science, energy storage and delivery, and membrane-based aqueous separations.

Jaden Stutts, undergraduate researcher

Jaden, a rising Junior who joined the lab in Spring 2020, is currently working towards completing her Departmental Honors Thesis on a project centered around the fabrication and characterization of poly(hydroxyethylmethacrylate) (pHEMA) and lignin soft composites (i.e., composite hydrogels), which have potential applications in biotechnologies such as wound dressing and drug delivery.

While prevalent in biomedical research, hydrogels comprised primarily of pHEMA are not mechanically robust and suffer from degradation issues, limiting their implementation in many applications. With the introduction of lignin, an abundant biopolymer that is a byproduct of the pulping and paper industry, we are able to tune both the mechanical and transport properties of the soft composites, creating materials with tailored functionality. Along with altering various synthesis parameters, Jaden will also investigate how the properties of the composite hydrogels change depending the source of the lignin – e.g., hardwood or softwood.

Alana Lesuer, undergraduate researcher, and Xueting Wang, Ph.D. student

Alana, a rising Senior in the program, is working with one of the graduate students in Prof. Davis’s lab on a project involving ionomer (i.e., polymers containing a fixed charge along the backbone) nanocomposites for use in vanadium redox flow batteries. Redox flow batteries, which can be thought of as large car batteries, have emerged as a promising electrical grid-scale energy storage technology due to their scalability.

However, the current state-of-the-art ionomer used to separate the liquid electrolytes in the battery suffers from issues related to electrolyte crossover, reducing the efficiency and lifetime of the battery. To address this issue, Alana will work to fabricate and characterize ionomers containing functionalized nanoparticles that have shown promise at addressing issues related to electrolyte crossover without compromising the attractive properties of these ionomer membranes.

Specifically, Alana will be synthesizing sulfonated poly(ether ether ketone) membranes containing silica nanoparticles with a wide range of surface functionalities. By varying the concentration and surface functionalization of the nanoparticles, the ion transport properties of the membranes can be significantly altered, ultimately leading to membranes with better performance properties than the current benchmark ionomers.

Both Jaden’s and Alana’s work are funded by a summer research grant through the Clemson University Creative Inquiry Program, as well as through an external grant from the Materials Assembly and Design Excellence in South Carolina (MADE in SC) Program titled, “Closing the Gap of Underrepresented Minorities and Women in Polymer-Related Research”.

Student researchers in catalysis to tackle greenhouse gas emissions and to produce clean energy

Case Sandor and John Yeagar are working on the project entitled “Catalysis for Carbon-Neutral space Exploration”, to produce propulsion fuel molecules through cathode reactions and life-supply O2 through anode reactions in separated streams.

Four student researchers engaged in catalytic materials and reaction engineering research at Dr. Ming Yang’s group have been recently recognized by the university and external funding agencies for their ongoing research work that aims to tackle climate change and to harvest clean energies.

Luis Morales and John Yeager, junior undergraduate students, were recently accepted into Clemson’s 2021 Summer Creative Inquiry program and given the accompanying Undergraduate Research Award (CI & UR Award). Luis and John, through both thermocatalytic and electrocatalytic approaches, will investigate how chemical engineers can significantly cut CO2 greenhouse gas emissions by turning the CO2 into value-added products through cost-effective environmentally benign catalytic reactions.

Case Sandor, senior undergraduate student, is a recipient of the Undergraduate Student Award from South Carolina NASA Space Grant Consortium. The agency funded Case for 400 hours of research to develop catalytic materials and electrochemical reactors that can convert CO2 in space and cabin into renewable fuels so as to empower deep space exploration. Case will also participate in outreach/public engagement activities during his award period to advocate research and science to public.

Ewa Chukwu and Luis Morales are undertaking a thermocatalytic approach in the project entitled “Catalysis for Clean Manufacturing”. Their research seeks to optimize the selective reduction of CO2 to value-added carbon-based chemicals as building blocks for various industrial processes.

Ewa Chukwu, a first-year international PhD student, has been selected to participate in the Student Program at the 2021 ARPA-E Energy Innovation Summit sponsored by the US Department of Energy. The 2021 Student Program will include the top graduate-level students, an engaging panel discussion regarding a career in energy, a Meet & Greet to speak with energy industries, and many opportunities to learn about cutting-edge energy initiatives.

Congratulations Case, Ewa, John, and Luis! Full speed ahead!

ChBE hosts its first virtual graduate symposium

Nick Gregorich and Molli Garifo

The department graduate symposium occurred on April 13, 2021 after not having a symposium in 2020 due to the pandemic. Planning a research symposium was a major hurdle for Nick Gregorich, Molli Garifo, and Mark Pitman. The CEGSO board devised an idea to utilize Google Sites for displaying the posters virtually. These personalized Google Sites allowed students to upload a poster, abstract, and photo of themselves displayable on the internet. In an effort to mirror an in-person poster symposium virtually, the students sat in individual zoom rooms for a virtual question and answer period while faculty and students could jump from room to room and view the students with their posters.

Zoom links were set up for each person so that judges were able to enter a zoom call and discuss the students’ posters with them. A Google Form was also set up for the judges to input their scoring. For the oral presentations, there was one zoom link that every participant was able to join and give their presentation. Even with some unforeseen technical difficulties, the symposium was an overall success and the event was able to get back on track very quickly with an engaged audience.

Our department would like to extend our thanks to Ph.D. students for their leadership as well as the Graduate Student Coordinator, Diana Stamey. We would also like to thank our faculty members who dedicated their time to serve as judges for the event.

1st Place Best Poster Presentation, Caleb Arp
2nd Place Poster Presentation, Graham Tindall & Nicholas Gregorich

 

 

 

 

 

 

 

1st Place Best Oral Presentation, Molly Wintenberg
2nd Place Oral Presentation, Jiarun Zhou
3rd Place Oral Presentation, James Foster

 

Dr. Jessica Larsen is the recipient of the 2021 Outstanding Woman Faculty Award

On April 26, Dr. Jessica Larsen was presented with the 2021 Outstanding Woman Faculty Award by Clemson University’s Commission on Women. She is adding this award to a growing list as Dr. Larsen recently received the 2021 National Science Foundation CAREER award and last year’s Bradley Mentoring Award.

Dr. Larsen leads a research program that centers on biomimetic and polymeric materials for drug delivery applications in neurodegenerative disease and other brain disorders. She is a passionate role model both at the university and in the broader community as an Assistant Coach for the Seneca High School Cross Country team.

As a faculty member of our department, Dr. Larsen aims to be an advocate for undergraduate woman as she continuously demonstrates a passion towards the advancement of female scientists. Since beginning as an Assistant Professor in 2017, Dr. Larsen has mentor a total of 44 undergraduate researchers with a high majority  (32) of those students being female scientists throughout multiple departments at the university. Dr. Larsen aims to provide her students with research independence so that they are able to gain confidence to present at national and international conferences and publish journal articles.

Dr. Larsen recently asked her students what they want their laboratory to be known for; Among the answers was not only producing high quality scientific products, but also being respectful, considerate members of society who value diversity and inclusion. This is evidence of the inclusive community that Dr. Larsen is trying to build in our department.

Congratulations, Dr. Larsen!

April 29, 2021, Seminar Series – Dr. Ana C. Alba-Rubio, The University of Toledo

Homogeneous and heterogeneous catalysis are fields that have been historically separated. Poly(styrenesulfonic acid) (PSSA) combines the advantages of both homogeneous and heterogeneous catalysis. PSSA is soluble in polar solvents; therefore, all acidic groups are readily accessible. In addition, the catalyst cannot be deactivated through coking because there is no surface for the carbonaceous species to be deposited. At the same time, PSSA, due to its high molecular weight, can be easily recovered by ultrafiltration for further utilization. PSSA has shown promising activity in reactions that require Brønsted acid sites, such as the hydrolysis of PET, dehydration of xylose to furfural, or biodiesel production from vegetable oils. We have recently added a second functionality to this polymer catalyst by the incorporation of AlCl3, and this led to a soluble and reusable catalyst with both Brønsted and Lewis acid sites (PSSA-AlCl3). This superacid catalyst was able to catalyze all the reactions involved in the one-pot synthesis of hydroxymethylfurfural (HMF) from glucose and directly from starch. HMF is a valuable platform chemical, and there is an enormous market for some of its derivatives, such as furandicarboxylic acid (FDCA) and adipic acid. This talk will also address the synthesis of inverse metal oxide-metal catalysts for the hydrogenation of CO2 and the addition of a sorbent component for the development of dual-function materials (CO2 capture & conversion). This one-pot approach could eliminate current energy-intensive and corrosive CO2 capture and storage processes while producing important commodity chemicals and fuels.

Dr. Ana C. Alba-Rubio is an assistant professor in the Department of Chemical Engineering at the University of Toledo. She received her B.S. in chemical engineering from the University of Malaga, Spain, in 2007. She then moved to the Institute of Catalysis and Petrochemistry (CSIC) in Madrid, Spain, to pursue her doctoral studies under the supervision of Dr. Manuel López Granados. As a doctoral student, she enjoyed two research stays in Brazil and the Netherlands. After receiving her Ph.D., Dr. Alba-Rubio joined the Department of Chemical and Biological Engineering at the University of Wisconsin-Madison to conduct postdoctoral research with Prof. James Dumesic and Prof. Manos Mavrikakis. She focused on the controlled synthesis of metal, bimetallic, and bifunctional catalysts for reactions of interest in biomass conversion and the direct synthesis of H2O2. In 2015, she joined the University of Toledo as a faculty, and her current research interests involve the rational design and synthesis of heterogeneous catalysts to provide fuels and materials sustainably and the development of nanomaterials for sensing applications. Dr. Alba-Rubio holds a CAREER Award from the National Science Foundation studying the development of dual-function materials for CO2 capture and conversion, the 2019 UToledo College of Engineering Excellence in Supervision of Undergraduate Research Award, and the 2018 UToledo Outstanding Scholarly and Creative Activity Award. She was also named to the 2020 Class of Influential Researchers by the Industrial & Engineering Chemistry Research journal and recognized as one of the 2021 Nanoscale Emerging Investigators. She currently serves as a director of the AIChE Catalysis and Reaction Engineering Division (CRE).

Unit Operations Laboratories Success in the Face of a Pandemic

The 2020 Spring semester started the same way one would remember every semester, with a mixture of new classes, familiar faces and places, and new challenges. Our department wouldn’t find out until March that those new challenges would include adjusting our teaching and learning methods in our Unit Operations lab while students and faculty were not allowed on campus.

Our faculty encountered a sudden dilemma of how to finish junior Unit Operations Laboratory experiments online in place of in person. Fortunately, 2 of the 4 experiments and the walkthrough for the 3rd experiment had been completed in person. With students sent home for the rest of the semester at Spring Break, department head Dr. Bruce, department technicians Messrs. Coburn and Marcengill, and faculty coaches Drs. Norfolk, Roat, and Thies scrambled to figure out how to get students through the remaining 2 experiments and associated reports by the end of the semester, entirely remotely.

The Spring semester ended with what could best be described as triage, as last-minute adjustments to the lab schedule were forced upon us. Plans for Unit Ops in the summer semester were scrapped, and the popular Study Abroad option at The Technical University of Denmark (DTU) was also canceled. This threatened the graduation plans of several juniors and seniors, particularly Co-Op students.

As summer progressed, it became more evident that our hopes of the pandemic lessening by the Fall semester were deluded as cases continued to surge throughout the country, especially after holiday gatherings. Starting in July, Dr. Norfolk directed the development of plans for multiple contingencies, including classes entirely online again, including all Unit Operations Lab classes; hybrid classes where some students would be allowed on campus; and resumption of “normal” operations with more accommodations for students physically challenged to navigate the Unit Ops Lab spaces.

With a passion for teaching the importance of safety, especially in the Unit Operations lab, Dr. Thies wrote a letter to Provost Jones requesting in-person Unit Operations Laboratory access sooner than the planned start to on-campus classes on September 21. Dr. Thies stressed that the safety of our engineers depended not only on keeping them safe during the pandemic but also on training them to be safe engineers in the future and that the experience in the Lab was vital for their training. All faculty and staff involved in Unit Operations Labs endorsed the letter.
While we were not granted an early start, we received permission to continue with in-person experiments, with appropriate testing and PPE, even if the campus was closed before completing all experiments.

The next challenge was keeping students safe when they were in the Lab. Our standard safety protocols, suitable for happier days, would not be sufficient for pandemic conditions. Face masks are currently mandatory inside all buildings on campus (and outside, when social distancing cannot be guaranteed). We also implemented temperature checks and a self-assessment at the entrance to the Lab. Further, a touchless hand sanitizer station, dubbed FAST (Foot-Actuated Sanitizer for Tigers), was created and placed at the entrance to the Lab.

To maintain distancing during the Lab, our traditional three- or four-person lab teams could not all go to the Lab simultaneously. Instead, groups were limited to two students in the Lab at a time, and we had a ‘shift change’ where students would change over personnel. While meeting the original intent of reducing the density of students in the Lab, this also resulted in significantly increased student engagement with the lab experiments and a new appreciation of the difficulty of writing a procedure. In the past, student participation rates varied, with certain students taking a more active role than others in their group. With the rotation of students throughout the lab period, all students had to engage actively in the lab activities. Further, they all gained an appreciation for the level of detail and planning put into a procedure that someone else, either a group member or a front-line operator, will be executing without supervision. The idea of a shift change of the student group may very well be implemented in future offerings of the Unit Ops lab course, even after the pandemic has passed.

The faculty took lessons from the Spring semester and implemented them into the plans and schedules for the Fall semester. Instead of conducting experiments throughout the semester, the faculty decided to emphasize hands-on lab activities and scheduled these topics into a short period earlier in the semester, which also had the added benefit of flexibility. As students in the course become infected or exposed to those who were, the faculty could rearrange the lab schedule, allowing for students to clear isolation and quarantine protocols and go into the Lab at a later date. This has only been possible because of the dedication of our lab technicians, Bill Coburn and Chad Marcengill, who worked nights and weekends to make sure the Unit Operations Lab experience would be everything we promised to the students.

All of these changes were important to conducting Lab in-person safely. However, the administration required all courses, including labs, to be offered to students who determined that they could not come to campus safely. Accommodating students who would never set foot in the Unit Operations Lab but still be required to write procedures, analyze results, and communicate their conclusions, became a significant effort.

At the suggestion of Dr. Bruce, there has been an extensive effort to create 3D models of each experimental apparatus available in the Unit Ops lab. A graduate student in Mechanical Engineering, Mihir Kale, as well as an undergraduate in Chemical Engineering, Matt “XV” Holmes, have created these models based on the equipment on the floor. These models allow students to virtually walk around the apparatus, trace the lines, and plan their experiments. When combined with a video of the faculty discussing the experiment and equipment (a recorded ‘walk through’ meeting), virtual students can plan and write procedures from the safety of their own home, wherever that might be. Students received support through virtual meetings with the faculty to answer questions.

The faculty are planning a comparison study of the effectiveness of these virtual tools. Student reports, including schematics and procedures, are available for classes in the past, which had access to the equipment and in-person walkthrough meetings. We intend to compare strategies generated by students with virtual tools and those created by students with only in-person tools to determine if students were equally able to demonstrate their mastery of the learning objectives. Our working hypothesis is that there will be no statistically significant difference between these students, which would support the use of virtual tools in the learning environment. Look for our analysis in a future edition of the Journal of Chemical Engineering Education.
With all of these safeguards in place and the adherence to the guidance of the university and other health officials, there were no documented cases of community spread that occurred in the Unit Ops Lab. While the pandemic is with us, we will continue our best efforts to safely train engineers on how to work in an industrial environment safely, and we will continue to look forward to the day that the sun shines on a mask-free Clemson, again!